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验证数据展示

  • WB analysis of NIH/3T3 using 25287-1-AP

    WB analysis of NIH/3T3 using 25287-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25287-1-AP (FER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 25287-1-AP

    IP experiment of MCF-7 using 25287-1-AP

    IP result of anti-FER (IP:25287-1-AP, 4ug; Detection:25287-1-AP 1:1000) with MCF-7 cells lysate 2400ug.

  • IHC staining of human kidney using 25287-1-AP

    IHC staining of human kidney using 25287-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25287-1-AP (FER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FER antibody included
  • IHC staining of human kidney using 25287-1-AP

    IHC staining of human kidney using 25287-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25287-1-AP (FER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FER antibody included
  • IF Staining of MCF-7 using 25287-1-AP

    IF Staining of MCF-7 using 25287-1-AP

    Immunofluorescent analysis of MCF-7 cells using 25287-1-AP (FER antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

FER Polyclonal antibody

FER Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 25287-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

FER, p94 Fer, Proto oncogene c Fer, TYK3, Tyrosine kinase 3, Tyrosine protein kinase Fer

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, A431 cells, HeLa cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy FER Ready-To-Use IHC Kit. FER primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

25287-1-AP targets FER in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag18173

Product name: Recombinant human FER protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 96-445 aa of BC017060

Sequence: PLHRLTMMIKDKQQVKKSYIGVHQQIEAEMIKVTKTELEKLKCSYRQLIKEMNSAKEKYKEALAKGKETEKAKERYDKATMKLHMLHNQYVLALKGAQLHQNQYYDITLPLLLDSLQKMQEEMIKALKGIFDEYSQITSLVTEEIVNVHKEIQMSVEQIDPSTEYNNFIDVHRTTAAKEQEIEFDTSLLEENENLQANEIMWNNLTAESLQVMLKTLAEELMQTQQMLLNKEEAVLELEKRIEESSETCEKKSDIVLLLSQKQALEELKQSVQQLRCTEAKFSAQKELLEQKVQENDGKEPPPVVNYEEDARSVTSMERKERLSKFESIRHSIAGIIRSPKSALGSSALS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fer (fps/fes related) tyrosine kinase
别名 FER, p94 Fer, Proto oncogene c Fer, TYK3, Tyrosine kinase 3, Tyrosine protein kinase Fer
计算分子量 822 aa, 95 kDa
观测分子量 95 kDa
GenBank蛋白编号BC017060
基因名称 FER
Gene ID (NCBI) 2241
RRIDAB_2880009
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16591
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FER(Tyrosine-protein kinase Fer) is also named as TYK3 and belongs to the protein kinase superfamily. Tyrosine-protein kinase acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. This protein has 3 isoforms produced by alternative promoter usage and alternative splicing.

实验方案

Product Specific Protocols
FC protocol for FER antibody 25287-1-APDownload protocol
IF protocol for FER antibody 25287-1-APDownload protocol
IHC protocol for FER antibody 25287-1-APDownload protocol
IP protocol for FER antibody 25287-1-APDownload protocol
WB protocol for FER antibody 25287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

BMC Cancer

The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.

Authors - Xue Yang
View Article
humanWB

J Biol Chem

Development of the non-receptor tyrosine kinase FER-targeting PROTACs as a potential strategy for antagonizing ovarian cancer cell motility and invasiveness

Authors - Yanchun Zhang
View Article
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