验证数据展示
经过测试的应用
| Positive IF-P detected in | Human liver cancer tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67075 targets Endoglin/CD105 in IF-P applications and shows reactivity with human samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag27837 Product name: Recombinant human Endoglin/CD105 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 33-221 aa of BC014271 Sequence: QPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCHLEGVAGHKEAHIL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | endoglin |
| 别名 | CD105, 2D5E8, END, Endoglin, ENG |
| 计算分子量 | 71 kDa |
| 观测分子量 | 95-100 kDa |
| GenBank蛋白编号 | BC014271 |
| 基因名称 | CD105 |
| Gene ID (NCBI) | 2022 |
| RRID | AB_2919417 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P17813 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Endoglin/CD105 antibody CL488-67075 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
