Endoglin/CD105 Monoclonal antibody
Endoglin/CD105 Monoclonal Antibody for FC, IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human and More (3)
Applications
FC, IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2D5E8
验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, HUVEC cells, human placenta tissue |
| Positive IHC detected in | human breast cancer tissue, human placenta tissue, human renal cell carcinoma tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue, human liver cancer tissue |
| Positive FC detected in | HUVEC cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:10000-1:40000 |
| Immunofluorescence (IF) | IF : 1:5000-1:20000 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67075-1-Ig targets Endoglin/CD105 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | FC, IF-P, IHC, WB, ELISA |
| Cited Applications | IF, FC, IHC |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Endoglin/CD105 fusion protein Ag27837 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | endoglin |
| Synonyms | CD105, END, endoglin, ENG, FLJ41744, HHT1, ORW, ORW1 |
| Calculated Molecular Weight | 71 kDa |
| Observed Molecular Weight | 95-100 kDa |
| GenBank Accession Number | BC014271 |
| Gene Symbol | ENG |
| Gene ID (NCBI) | 2022 |
| RRID | AB_2882383 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P17813 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Endoglin/CD105 antibody 67075-1-Ig | Download protocol |
| IHC protocol for Endoglin/CD105 antibody 67075-1-Ig | Download protocol |
| IF protocol for Endoglin/CD105 antibody 67075-1-Ig | Download protocol |
| FC protocol for Endoglin/CD105 antibody 67075-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Stem Cell Res Ther Glioma-associated mesenchymal stem cells-mediated PD-L1 expression is attenuated by Ad5-Ki67/IL-15 in GBM treatment. | ||
Cell Tissue Res A histological study of vascular wall resident stem cells in venous malformations | ||
Dis Markers Long Noncoding RNA SBF2-AS1 Promotes Abdominal Aortic Aneurysm Formation through the miRNA-520f-3p/SMARCD1 Axis | ||
Front Bioeng Biotechnol Quiescence preconditioned nucleus pulposus stem cells alleviate intervertebral disc degeneration by enhancing cell survival via adaptive metabolism pattern in rats |
