验证数据展示
经过测试的应用
Positive IF/ICC detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy BCL10 Ready-To-Use IHC Kit. BCL10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17732-1-AP
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-17732 targets BCL10 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | BCL10 fusion protein Ag12170 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | B-cell CLL/lymphoma 10 |
别名 | B cell CLL/lymphoma 10, B cell lymphoma/leukemia 10, BCL10, c E10, CARD like apoptotic protein, CARMEN, cCARMEN, Cellular E10, Cellular homolog of vCARMEN, CIPER, CLAP, hCLAP, mE10 |
计算分子量 | 233 aa, 26 kDa |
观测分子量 | 30 kDa |
GenBank蛋白编号 | BC053617 |
基因名称 | BCL10 |
Gene ID (NCBI) | 8915 |
RRID | AB_2919635 |
偶联类型 | CoraLite®555 Fluorescent Dye |
最大激发/发射波长 | 557 nm / 570nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O95999 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The gene encoding BCL10 was identified by its recurrent translocation t(1;14)(p22;q32) in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. BCL10 contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB.
实验方案
Product Specific Protocols | |
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IF protocol for CL555 BCL10 antibody CL555-17732 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |