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验证数据展示

  • FC experiment of Jurkat using CL647-17732

    FC experiment of Jurkat using CL647-17732

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human BCL10 (CL647-17732) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO验证

CoraLite® Plus 647-conjugated BCL10 Polyclonal antibody

BCL10 Polyclonal Antibody for FC (Intra)
Cat No. CL647-17732

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

CARD-containing molecule enhancing NF-kappa-B, c E10, Bcl-10, Bcl 10, B-cell lymphoma/leukemia 10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy BCL10 Ready-To-Use IHC Kit. BCL10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17732-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-17732 targets BCL10 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12170

Product name: Recombinant human BCL10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-233 aa of BC053617

Sequence: MEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTEEISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQKITDEVLKLRNIKLEHLKGLKCSSCEPFPDGATNNLSRSNSDESNFSEKLRASTVMYHPEGESSTTPFFSTNSSLNLPVLEVGRTENTIFSSTTLPRPGDPGAPPLPPDLQLEEEGTCANSSEMFLPLRSRTVSRQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 B-cell CLL/lymphoma 10
别名 CARD-containing molecule enhancing NF-kappa-B, c E10, Bcl-10, Bcl 10, B-cell lymphoma/leukemia 10
计算分子量 233 aa, 26 kDa
观测分子量 30 kDa
GenBank蛋白编号BC053617
基因名称 BCL10
Gene ID (NCBI) 8915
RRIDAB_2934928
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95999
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The gene encoding BCL10 was identified by its recurrent translocation t(1;14)(p22;q32) in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. BCL10 contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 BCL10 antibody CL647-17732Download protocol
Standard Protocols
Click here to view our Standard Protocols