• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

BCL10 Polyclonal antibody

BCL10 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 17732-1-AP

Print datasheet

Synonyms

B cell CLL/lymphoma 10, B cell lymphoma/leukemia 10, BCL10, c E10, CARD like apoptotic protein, CARMEN, cCARMEN, Cellular E10, Cellular homolog of vCARMEN, CIPER, CLAP, hCLAP, mE10

验证数据展示

  • WB analysis using 17732-1-AP

    WB analysis using 17732-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17732-1-AP (BCL10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 17732-1-AP

    WB analysis of K-562 using 17732-1-AP

    WB result of BCL10 antibody (17732-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL10 transfected K-562 cells.

  • IHC staining of human breast cancer using 17732-1-AP

    IHC staining of human breast cancer using 17732-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17732-1-AP (BCL10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCL10 antibody included
  • IHC staining of human breast cancer using 17732-1-AP

    IHC staining of human breast cancer using 17732-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17732-1-AP (BCL10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCL10 antibody included
  • FC experiment of Jurkat using 17732-1-AP

    FC experiment of Jurkat using 17732-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human BCL10 (17732-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy BCL10 Ready-To-Use IHC Kit. BCL10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17732-1-AP targets BCL10 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen BCL10 fusion protein Ag12170 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name B-cell CLL/lymphoma 10
Synonyms B cell CLL/lymphoma 10, B cell lymphoma/leukemia 10, BCL10, c E10, CARD like apoptotic protein, CARMEN, cCARMEN, Cellular E10, Cellular homolog of vCARMEN, CIPER, CLAP, hCLAP, mE10
Calculated Molecular Weight 233 aa, 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC053617
Gene Symbol BCL10
Gene ID (NCBI) 8915
RRIDAB_2064860
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95999
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The gene encoding BCL10 was identified by its recurrent translocation t(1;14)(p22;q32) in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. BCL10 contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB.

实验方案

Product Specific Protocols
WB protocol for BCL10 antibody 17732-1-APDownload protocol
IHC protocol for BCL10 antibody 17732-1-APDownload protocol
FC protocol for BCL10 antibody 17732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols