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CoraLite® Plus 488-conjugated Annexin A11 Monoclonal antibody

Annexin A11 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A3C4

Cat No : CL488-68089

Print datasheet

Synonyms

56 kDa autoantigen, Annexin 11, annexin A11, Annexin XI, ANX11, ANXA11, CAP 50, CAP50

验证数据展示

  • IF Staining of HeLa using CL488-68089

    IF Staining of HeLa using CL488-68089

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Annexin A11 antibody (CL488-68089, Clone: 1A3C4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-68089

    FC experiment of HeLa using CL488-68089

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Annexin A11 (CL488-68089, Clone:1A3C4) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HEK-293T using CL488-68089

    FC experiment of HEK-293T using CL488-68089

    1X10^6 HEK-293T cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Annexin A11 (CL488-68089, Clone:1A3C4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells, HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy ANXA11 Ready-To-Use IHC Kit. ANXA11 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68089-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68089 targets Annexin A11 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen Annexin A11 fusion protein Ag31839 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name annexin A11
Synonyms 56 kDa autoantigen, Annexin 11, annexin A11, Annexin XI, ANX11, ANXA11, CAP 50, CAP50
Calculated Molecular Weight 56 kDa
GenBank Accession NumberBC007564
Gene Symbol ANXA11
Gene ID (NCBI) 311
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP50995
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling. Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Annexin A11 antibody CL488-68089Download protocol
FC protocol for CL Plus 488 Annexin A11 antibody CL488-68089Download protocol
Standard Protocols
Click here to view our Standard Protocols