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Annexin A11 Monoclonal antibody

Annexin A11 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A3C4

Cat No : 68089-1-Ig

Print datasheet

Synonyms

56 kDa autoantigen, Annexin 11, annexin A11, Annexin XI, ANX11, ANXA11, CAP 50, CAP50

验证数据展示

  • WB analysis using 68089-1-Ig

    WB analysis using 68089-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68089-1-Ig (Annexin A11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human lung cancer using 68089-1-Ig

    IHC staining of human lung cancer using 68089-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA11 antibody included
  • IHC staining of human lung cancer using 68089-1-Ig

    IHC staining of human lung cancer using 68089-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANXA11 antibody included
  • IF Staining of HeLa using 68089-1-Ig

    IF Staining of HeLa using 68089-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A11 antibody (68089-1-Ig, Clone: 1A3C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HEK-293T using 68089-1-Ig

    FC experiment of HEK-293T using 68089-1-Ig

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human Annexin A11 (68089-1-Ig, Clone:1A3C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HeLa using 68089-1-Ig

    FC experiment of HeLa using 68089-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Annexin A11 (68089-1-Ig, Clone:1A3C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA431 cells, A549 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig heart tissue, rabbit heart
Positive IHC detected inhuman lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHEK-293T cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy ANXA11 Ready-To-Use IHC Kit. ANXA11 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68089-1-Ig targets Annexin A11 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat, rabbit, pig
Cited Reactivitymouse
Immunogen Annexin A11 fusion protein Ag31839 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name annexin A11
Synonyms 56 kDa autoantigen, Annexin 11, annexin A11, Annexin XI, ANX11, ANXA11, CAP 50, CAP50
Calculated Molecular Weight 56 kDa
GenBank Accession NumberBC007564
Gene Symbol ANXA11
Gene ID (NCBI) 311
RRIDAB_2918826
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50995
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling. Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer.

实验方案

Product Specific Protocols
WB protocol for Annexin A11 antibody 68089-1-IgDownload protocol
IHC protocol for Annexin A11 antibody 68089-1-IgDownload protocol
IF protocol for Annexin A11 antibody 68089-1-IgDownload protocol
FC protocol for Annexin A11 antibody 68089-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell Proteomics

FMRP long-range transport and degradation are mediated by Dynlrb1 in sensory neurons

Authors - Sara Emad El-Agamy
View Article