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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68089-1-Ig (same clone as 68089-1-PBS)

    WB analysis using 68089-1-Ig (same clone as 68089-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68089-1-Ig (Annexin A11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68089-1-Ig (same clone as 68089-1-PBS)

    IHC staining of human lung cancer using 68089-1-Ig (same clone as 68089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA11 antibody included
  • IHC staining of human lung cancer using 68089-1-Ig (same clone as 68089-1-PBS)

    IHC staining of human lung cancer using 68089-1-Ig (same clone as 68089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA11 antibody included
  • FC experiment of HEK-293T using 68089-1-Ig (same clone as 68089-1-PBS)

    FC experiment of HEK-293T using 68089-1-Ig (same clone as 68089-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human Annexin A11 (68089-1-Ig, Clone:1A3C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68089-1-Ig (same clone as 68089-1-PBS)

    FC experiment of HeLa using 68089-1-Ig (same clone as 68089-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Annexin A11 (68089-1-Ig, Clone:1A3C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68089-1-Ig (same clone as 68089-1-PBS)

    IF Staining of HeLa using 68089-1-Ig (same clone as 68089-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A11 antibody (68089-1-Ig, Clone: 1A3C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

Annexin A11 Monoclonal antibody, PBS Only

Annexin A11 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 68089-1-PBS

产品说明书

CloneNo. 1A3C4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

ANXA11, 1A3C4, 56 kDa autoantigen, Annexin 11, Annexin XI

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ANXA11 Ready-To-Use IHC Kit. ANXA11 primary antibody included.

产品信息

68089-1-PBS targets Annexin A11 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Annexin A11 fusion protein Ag31839 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 annexin A11
别名 ANXA11, 1A3C4, 56 kDa autoantigen, Annexin 11, Annexin XI
计算分子量 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC007564
基因名称 Annexin A11
Gene ID (NCBI) 311
RRIDAB_2918826
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP50995
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling (PMID: 31610865). ANXA11, an RNA granule-associated phosphoinositide-binding protein, acts as a molecular tether between RNA granules and lysosomes (PMID: 31539493). Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer. Annexin A11, is involved in a variety of cellular functions including mRNA transport and translation, endocytosis, exocytosis, and plasma membrane repair (PMID: 38896345).