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验证数据展示

  • IF Staining of HepG2 using CL594-66600

    IF Staining of HepG2 using CL594-66600

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 ATPB antibody (CL594-66600, Clone: 1A11B9 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-66600

    FC experiment of HepG2 using CL594-66600

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human ATPB (CL594-66600, Clone:1A11B9) (red), or 0.8 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated ATPB Monoclonal antibody

ATPB Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66600

产品说明书

CloneNo. 1A11B9
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra) and More (1)

别名

ATPSB, ATPMB, ATP5F1B, ATP5B, ATP synthase F1 subunit beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ATP5B Ready-To-Use IHC Kit. ATP5B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66600-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL594-66600 targets ATPB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
免疫原 ATPB fusion protein Ag11177 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
别名 ATPSB, ATPMB, ATP5F1B, ATP5B, ATP synthase F1 subunit beta
计算分子量 529 aa, 57 kDa
观测分子量45-57 kDa
GenBank蛋白编号BC016512
基因名称 ATPB
Gene ID (NCBI) 506
RRIDAB_2920015
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06576
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.

实验方案

Product Specific Protocols
IF protocol for CL594 ATPB antibody CL594-66600Download protocol
FC protocol for CL594 ATPB antibody CL594-66600Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Exp Ther Med

Targeting translocator protein protects against myocardial ischemia/reperfusion injury by alleviating mitochondrial dysfunction

Authors - Chenghao Wen
View Article