验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ATP5B Ready-To-Use IHC Kit. ATP5B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66600-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66600 targets ATPB in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ATPB fusion protein Ag11177 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide |
| 别名 | ATPSB, ATPMB, ATP5F1B, ATP5B, ATP synthase F1 subunit beta |
| 计算分子量 | 529 aa, 57 kDa |
| 观测分子量 | 45-57 kDa |
| GenBank蛋白编号 | BC016512 |
| 基因名称 | ATPB |
| Gene ID (NCBI) | 506 |
| RRID | AB_3672917 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P06576 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ATPB antibody CL488-66600 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |