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验证数据展示

  • IF Staining of HeLa using CL488-12640

    IF Staining of HeLa using CL488-12640

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 golgin 97 antibody (CL488-12640) at dilution of 1:400, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-12640

    FC experiment of HeLa using CL488-12640

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human golgin 97 (CL488-12640) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated golgin 97 Polyclonal antibody

golgin 97 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-12640

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

GOLGA1, Golgin-97, Golgin subfamily A member 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12640-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12640 targets golgin 97 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen golgin 97 fusion protein Ag3335 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name golgi autoantigen, golgin subfamily a, 1
Synonyms GOLGA1, Golgin-97, Golgin subfamily A member 1
Calculated Molecular Weight 767 aa, 88 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC032853
Gene Symbol golgin 97
Gene ID (NCBI) 2800
RRIDAB_3083930
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92805
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa. IF result shows strong Gogli staining.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 golgin 97 antibody CL488-12640Download protocol
FC protocol for CL Plus 488 golgin 97 antibody CL488-12640Download protocol
Standard Protocols
Click here to view our Standard Protocols