golgin 97 Polyclonal antibody

golgin 97 Polyclonal Antibody for FC, IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

FC, IF/ICC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 12640-1-AP

Print datasheet

Synonyms

GOLGA1, Golgi marker, golgin 97, Golgin subfamily A member 1



经过测试的应用

Positive WB detected inHEK-293 cells, SMMC-7721 cells, HeLa cells, HepG2 cells
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12640-1-AP targets golgin 97 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, monkey
Immunogen golgin 97 fusion protein Ag3335 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name golgi autoantigen, golgin subfamily a, 1
Synonyms GOLGA1, Golgi marker, golgin 97, Golgin subfamily A member 1
Calculated Molecular Weight 767 aa, 88 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC032853
Gene Symbol GOLGA1
Gene ID (NCBI) 2800
RRIDAB_2115315
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92805
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa, and Catalog#12640-1-AP specially recognises the 97 kDa protein. IF result shows strong Gogli staining.

实验方案

Product Specific Protocols
WB protocol for golgin 97 antibody 12640-1-APDownload protocol
IF protocol for golgin 97 antibody 12640-1-APDownload protocol
FC protocol for golgin 97 antibody 12640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Commun

Depletion of mitochondrial methionine adenosyltransferase α1 triggers mitochondrial dysfunction in alcohol-associated liver disease.

Authors - Lucía Barbier-Torres
human,mouseIF

Redox Biol

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis

Authors - Mengyue You
humanWB

Dev Cell

GRASP55 Senses Glucose Deprivation through O-GlcNAcylation to Promote Autophagosome-Lysosome Fusion.

Authors - Xiaoyan Zhang
humanWB

Cell Rep

CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth

Authors - Shasha Yin
humanIF

Cell Chem Biol

Artemisinin inhibits NRas palmitoylation by targeting the protein acyltransferase ZDHHC6.

Authors - Nan Qiu
humanWB,IF

Mol Metab

CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.

Authors - Han Zeng