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验证数据展示

  • FC experiment of K-562 using PE-67447

    FC experiment of K-562 using PE-67447

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug PE Anti-Human c-MYC (PE-67447, Clone:3D9C12) (red), or 0.4 ug PE Mouse IgG1 Isotype Control (MOPC-21) (PE-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

PE-conjugated c-MYC Monoclonal antibody

c-MYC Monoclonal Antibody for FC (Intra)
Cat No. PE-67447

产品说明书

CloneNo. 3D9C12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

MYC, 3D9C12, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  PE
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inK-562 cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-67447 targets c-MYC in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17519

Product name: Recombinant human MYC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 16-454 aa of BC000141

Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 v-myc myelocytomatosis viral oncogene homolog (avian)
别名 MYC, 3D9C12, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39
计算分子量 49 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC000141
基因名称 MYC
Gene ID (NCBI) 4609
偶联类型 PE Fluorescent Dye
最大激发/发射波长496 nm, 565 nm / 578 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP01106
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.

实验方案

Product Specific Protocols
FC protocol for PE c-MYC antibody PE-67447Download protocol
Standard Protocols
Click here to view our Standard Protocols