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CoraLite®555-conjugated c-MYC Monoclonal antibody

c-MYC Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3D9C12

Cat No : CL555-67447

Print datasheet

Synonyms

bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64

验证数据展示

  • FC experiment of K-562 using CL555-67447

    FC experiment of K-562 using CL555-67447

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human c-MYC (CL555-67447, Clone:3D9C12) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 67447-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67447 targets c-MYC in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity Human, mouse, rat
Immunogen c-MYC fusion protein Ag17519 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Synonyms bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC000141
Gene Symbol MYC
Gene ID (NCBI) 4609
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP01106
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.

实验方案

Product Specific Protocols
FC protocol for CL555 c-MYC antibody CL555-67447Download protocol
Standard Protocols
Click here to view our Standard Protocols