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验证数据展示

  • IF Staining of HepG2 using CL488-10936

    IF Staining of HepG2 using CL488-10936

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 YWHAB antibody (CL488-10936) at dilution of 1:200.

CoraLite® Plus 488-conjugated YWHAB Polyclonal antibody

YWHAB Polyclonal Antibody for IF/ICC
Cat No. CL488-10936

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

14 3 3 protein beta/alpha, 14 3 3,, GW128, HS1, KCIP 1, Protein 1054, YWHAB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy YWHAB Ready-To-Use IHC Kit. YWHAB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10936-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10936 targets YWHAB in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 YWHAB fusion protein Ag1383 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
别名 14 3 3 protein beta/alpha, 14 3 3,, GW128, HS1, KCIP 1, Protein 1054, YWHAB
计算分子量 28 kDa
GenBank蛋白编号BC010352
基因名称 YWHAB
Gene ID (NCBI) 7529
RRIDAB_3083888
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31946
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YWHAB (also known as 14-3-3 beta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised agaist the N-terminal region of human YWHAB.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 YWHAB antibody CL488-10936Download protocol
Standard Protocols
Click here to view our Standard Protocols