验证数据展示
经过测试的应用
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| ELISA | See 1 publications below |
产品信息
10936-1-AP targets YWHAB in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | ELISA |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 | YWHAB fusion protein Ag1383 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide |
| 别名 | HS1, GW128, 14-3-3 protein beta/alpha, N-terminally processed, 14-3-3 protein beta/alpha, 14 3 3, |
| 计算分子量 | 28 kDa |
| GenBank蛋白编号 | BC010352 |
| 基因名称 | YWHAB |
| Gene ID (NCBI) | 7529 |
| RRID | AB_2217489 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P31946 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YWHAB (also known as 14-3-3 beta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised agaist the N-terminal region of human YWHAB.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for YWHAB antibody 10936-1-AP | Download protocol |
| IF protocol for YWHAB antibody 10936-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
