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CoraLite® Plus 488-conjugated YTHDC1 Monoclonal antibody

YTHDC1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F5G11

Cat No : CL488-67911

Print datasheet

Synonyms

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1

验证数据展示

  • FC experiment of A431 using CL488-67911

    FC experiment of A431 using CL488-67911

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human YTHDC1 (CL488-67911, Clone:1F5G11) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67911-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67911 targets YTHDC1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen YTHDC1 fusion protein Ag6289 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name YTH domain containing 1
Synonyms KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1
Calculated Molecular Weight 85 kDa
Observed Molecular Weight~100 kDa
GenBank Accession NumberBC053863
Gene Symbol YTHDC1
Gene ID (NCBI) 91746
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96MU7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 YTHDC1 antibody CL488-67911Download protocol
Standard Protocols
Click here to view our Standard Protocols