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YTHDC1 Monoclonal antibody

YTHDC1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F5G11

Cat No : 67911-1-Ig

Print datasheet

Synonyms

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1

验证数据展示

  • WB analysis using 67911-1-Ig

    WB analysis using 67911-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67911-1-Ig (YTHDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • IHC staining of human breast cancer using 67911-1-Ig

    IHC staining of human breast cancer using 67911-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67911-1-Ig

    IHC staining of human breast cancer using 67911-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 67911-1-Ig

    IHC staining of human cervical cancer using 67911-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 67911-1-Ig

    IHC staining of mouse testis using 67911-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 67911-1-Ig

    IHC staining of mouse testis using 67911-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inLNCaP cells, MDA-MB-231 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67911-1-Ig targets YTHDC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen YTHDC1 fusion protein Ag6289 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name YTH domain containing 1
Synonyms KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1
Calculated Molecular Weight 85 kDa
Observed Molecular Weight~100 kDa
GenBank Accession NumberBC053863
Gene Symbol YTHDC1
Gene ID (NCBI) 91746
RRIDAB_2918666
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96MU7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).

实验方案

Product Specific Protocols
WB protocol for YTHDC1 antibody 67911-1-IgDownload protocol
IHC protocol for YTHDC1 antibody 67911-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols