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CoraLite®594-conjugated XRCC2 Monoclonal antibody

XRCC2 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D9A1

Cat No : CL594-66652

Print datasheet

Synonyms

DKFZp781P0919, DNA repair protein XRCC2, XRCC2

验证数据展示

  • IF Staining of L02 using CL594-66652

    IF Staining of L02 using CL594-66652

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using CoraLite®594-conjugated XRCC2 antibody (CL594-66652, Clone: 3D9A1 ) at dilution of 1:100, CL488-Phalloidin (green).

  • FC experiment of HepG2 using CL594-66652

    FC experiment of HepG2 using CL594-66652

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human XRCC2 (CL594-66652, Clone:3D9A1) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inL02 cells, HepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66652-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66652 targets XRCC2 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human
Immunogen XRCC2 fusion protein Ag14421 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name X-ray repair complementing defective repair in Chinese hamster cells 2
Synonyms DKFZp781P0919, DNA repair protein XRCC2, XRCC2
Calculated Molecular Weight 280 aa, 32 kDa
GenBank Accession NumberBC042137
Gene Symbol XRCC2
Gene ID (NCBI) 7516
RRIDAB_2883610
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43543
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway.

实验方案

Product Specific Protocols
IF protocol for CL594 XRCC2 antibody CL594-66652Download protocol
FC protocol for CL594 XRCC2 antibody CL594-66652Download protocol
Standard Protocols
Click here to view our Standard Protocols