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验证数据展示

  • FC experiment of HepG2 using CL555-66652

    FC experiment of HepG2 using CL555-66652

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human XRCC2 (CL555-66652, Clone:3D9A1) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HepG2 using CL555-66652

    IF Staining of HepG2 using CL555-66652

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 XRCC2 antibody (CL555-66652, Clone: 3D9A1 ) at dilution of 1:200, CL488-phalloidin (green).

CoraLite®555-conjugated XRCC2 Monoclonal antibody

XRCC2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-66652

产品说明书

CloneNo. 3D9A1

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

3D9A1, DNA repair protein XRCC2, X-ray repair cross-complementing protein 2

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®555
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66652-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66652 targets XRCC2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14421

Product name: Recombinant human XRCC2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-280 aa of BC042137

Sequence: MCSAFHRAESGTELLARLEGRSSLKEIEPNLFADEDSPVHGDILEFHGPEGTGKTEMLYHLTARCILPKSEGGLEVEVLFIDTDYHFDMLRLVTILEHRLSQSSEEIIKYCLGRFFLVYCSSSTHLLLTLYSLESMFCSHPSLCLLILDSLSAFYWIDRVNGGESVNLQESTLRKCSQCLEKLVNDYRLVLFATTQTIMQKASSSSEEPSHASRRLCDVDIDYRPYLCKAWQQLVKHRMFFSKQDDSQSSNQFSLVSRCLKSNSLKKHFFIIGESGVEFC

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 X-ray repair complementing defective repair in Chinese hamster cells 2
别名 3D9A1, DNA repair protein XRCC2, X-ray repair cross-complementing protein 2
计算分子量 280 aa, 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC042137
基因名称 XRCC2
Gene ID (NCBI) 7516
RRIDAB_3084627
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43543
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway.

实验方案

Product Specific Protocols
FC protocol for CL555 XRCC2 antibody CL555-66652Download protocol
IF protocol for CL555 XRCC2 antibody CL555-66652Download protocol
Standard Protocols
Click here to view our Standard Protocols
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