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验证数据展示

  • WB analysis of HEK-293 using 66652-1-Ig

    WB analysis of HEK-293 using 66652-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66652-1-Ig (XRCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66652-1-Ig

    IF Staining of HepG2 using 66652-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC2 antibody (66652-1-Ig, Clone: 3D9A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HepG2 using 66652-1-Ig

    IF Staining of HepG2 using 66652-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC2 antibody (66652-1-Ig, Clone: 3D9A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

  • FC experiment of HepG2 using 66652-1-Ig

    FC experiment of HepG2 using 66652-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XRCC2 (66652-1-Ig, Clone:3D9A1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

XRCC2 Monoclonal antibody

XRCC2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 66652-1-Ig

产品说明书

CloneNo. 3D9A1

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

3D9A1, DNA repair protein XRCC2, X-ray repair cross-complementing protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Raji cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66652-1-Ig targets XRCC2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14421

Product name: Recombinant human XRCC2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-280 aa of BC042137

Sequence: MCSAFHRAESGTELLARLEGRSSLKEIEPNLFADEDSPVHGDILEFHGPEGTGKTEMLYHLTARCILPKSEGGLEVEVLFIDTDYHFDMLRLVTILEHRLSQSSEEIIKYCLGRFFLVYCSSSTHLLLTLYSLESMFCSHPSLCLLILDSLSAFYWIDRVNGGESVNLQESTLRKCSQCLEKLVNDYRLVLFATTQTIMQKASSSSEEPSHASRRLCDVDIDYRPYLCKAWQQLVKHRMFFSKQDDSQSSNQFSLVSRCLKSNSLKKHFFIIGESGVEFC

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 X-ray repair complementing defective repair in Chinese hamster cells 2
别名 3D9A1, DNA repair protein XRCC2, X-ray repair cross-complementing protein 2
计算分子量 280 aa, 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC042137
基因名称 XRCC2
Gene ID (NCBI) 7516
RRIDAB_2882009
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43543
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway.

实验方案

Product Specific Protocols
IF protocol for XRCC2 antibody 66652-1-IgDownload protocol
WB protocol for XRCC2 antibody 66652-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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