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验证数据展示

  • IF Staining of U-937 using CL488-66175

    IF Staining of U-937 using CL488-66175

    Immunofluorescent analysis of (4% PFA) fixed U-937 cells using CoraLite® Plus 488 Vitamin D binding protein antibody (CL488-66175, Clone: 1E4D10 ) at dilution of 1:100.

  • IF Staining of HepG2 using CL488-66175

    IF Staining of HepG2 using CL488-66175

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Vitamin D binding protein antibody (CL488-66175, Clone: 1E4D10 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Vitamin D binding protein Monoclonal antibody

Vitamin D binding protein Monoclonal Antibody for IF/ICC
Cat No. CL488-66175

产品说明书

CloneNo. 1E4D10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC

别名

GC, VDBP,GC, Gc globulin, DBP-maf, DBP/GC

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inU-937 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GC/VTDB Ready-To-Use IHC Kit. GC/VTDB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66175-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66175 targets Vitamin D binding protein in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag9803

Product name: Recombinant human VDBP,GC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 120-474 aa of BC057228

Sequence: KLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKEYANQFMWEYSTNYGQAPLSLLVSYTKSYLSMVGSCCTSASPTVCFLKERLQLKHLSLLTTLSNRVCSQYAAYGEKKSRLSNLIKLAQKVPTADLEDVLPLAEDITNILSKCCESASEDCMAKELPEHTVKLCDNLSTKNSKFEDCCQEKTAMDVFVCTYFMPAAQLPELPDVELPTNKDVCDPGNTKVMDKYTFELSRRTHLPEVFLSKVLEPTLKSLGECCDVEDSTTCFNAKGPLLKKELSSFIDKGQELCADYSENTFTEYKKKLAERLKAKLPDATPTELAKLVNKRSDFASNCCSINSPPLYCDSEIDAELKNIL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 group-specific component (vitamin D binding protein)
别名 GC, VDBP,GC, Gc globulin, DBP-maf, DBP/GC
计算分子量 474 aa, 53 kDa
观测分子量 52-58 kDa
GenBank蛋白编号BC057228
基因名称 Vitamin D binding protein
Gene ID (NCBI) 2638
RRIDAB_3672907
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP02774
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Vitamin D binding protein antibody CL488-66175Download protocol
Standard Protocols
Click here to view our Standard Protocols
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