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验证数据展示

  • WB analysis of human testis using 66175-1-Ig

    WB analysis of human testis using 66175-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66175-1-Ig (Vitamin D binding protein antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66175-1-Ig

    IHC staining of human liver using 66175-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GC/VTDB antibody included
  • IHC staining of human colon cancer using 66175-1-Ig

    IHC staining of human colon cancer using 66175-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GC/VTDB antibody included
  • IHC staining of human liver cancer using 66175-1-Ig

    IHC staining of human liver cancer using 66175-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GC/VTDB antibody included
  • IF Staining of HepG2 using 66175-1-Ig

    IF Staining of HepG2 using 66175-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Vitamin D binding protein antibody (66175-1-Ig, Clone: 1E4D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of U-937 using 66175-1-Ig

    FC experiment of U-937 using 66175-1-Ig

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human Vitamin D binding protein (66175-1-Ig, Clone:1E4D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Vitamin D binding protein Monoclonal antibody

Vitamin D binding protein Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66175-1-Ig

产品说明书

CloneNo. 1E4D10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

GC, VDBP,GC, DBP, DBP/GC, DBP-maf

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman liver tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inU-937 cells
Planning an IHC experiment? We recommend our IHCeasy GC/VTDB Ready-To-Use IHC Kit. GC/VTDB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66175-1-Ig targets Vitamin D binding protein in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Vitamin D binding protein fusion protein Ag9803 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 group-specific component (vitamin D binding protein)
别名 GC, VDBP,GC, DBP, DBP/GC, DBP-maf
计算分子量 474 aa, 53 kDa
观测分子量 52-58 kDa
GenBank蛋白编号BC057228
基因名称 Vitamin D binding protein
Gene ID (NCBI) 2638
RRIDAB_2881570
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02774
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.

实验方案

Product Specific Protocols
WB protocol for Vitamin D binding protein antibody 66175-1-IgDownload protocol
IHC protocol for Vitamin D binding protein antibody 66175-1-IgDownload protocol
IF protocol for Vitamin D binding protein antibody 66175-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols