• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Vitamin D binding protein Monoclonal antibody

Vitamin D binding protein Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

FC, WB, ELISA and More (4)

Conjugate

Unconjugated

CloneNo.

1E4D10

Cat No : 66175-1-Ig

Print datasheet

Synonyms

DBP, DBP/GC, GC, Gc globulin, VDB, VDBG, VDBP, VDBP,GC, Vitamin D binding protein

验证数据展示

  • WB analysis of human testis using 66175-1-Ig

    WB analysis of human testis using 66175-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66175-1-Ig (Vitamin D binding protein antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of U-937 using 66175-1-Ig

    FC experiment of U-937 using 66175-1-Ig

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human Vitamin D binding protein (66175-1-Ig, Clone:1E4D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inhuman testis tissue
Positive FC detected inU-937 cells
Planning an IHC experiment? We recommend our IHCeasy GC/VTDB Ready-To-Use IHC Kit. GC/VTDB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66175-1-Ig targets Vitamin D binding protein in WB, IF, FC, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications FC, WB, ELISA
Cited ApplicationsWB, IF, IHC, CoIP, ChIP
Tested Reactivity human
Cited Reactivitymouse
Immunogen Vitamin D binding protein fusion protein Ag9803 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name group-specific component (vitamin D binding protein)
Synonyms DBP, DBP/GC, GC, Gc globulin, VDB, VDBG, VDBP, VDBP,GC, Vitamin D binding protein
Calculated Molecular Weight 474 aa, 53 kDa
Observed Molecular Weight 52-58 kDa
GenBank Accession NumberBC057228
Gene Symbol GC
Gene ID (NCBI) 2638
RRIDAB_2881570
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02774
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.

实验方案

Product Specific Protocols
WB protocol for Vitamin D binding protein antibody 66175-1-IgDownload protocol
FC protocol for Vitamin D binding protein antibody 66175-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC,IF,WB,CoIP,ChIP

Theranostics

Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma

Authors - Lu-Ning Qin
View Article