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验证数据展示

  • IF Staining of HeLa using CL488-67321

    IF Staining of HeLa using CL488-67321

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 USP8 antibody (CL488-67321, Clone: 1E12E3 ) at dilution of 1:200.

  • FC experiment of Hela using CL488-67321

    FC experiment of Hela using CL488-67321

    1X10^6 Hela cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human USP8 (CL488-67321, Clone:1E12E3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated USP8 Monoclonal antibody

USP8 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67321

产品说明书

CloneNo. 1E12E3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

1E12E3, EC:3.4.19.12, hUBPy, HumORF8, KIAA0055

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy USP8 Ready-To-Use IHC Kit. USP8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67321-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67321 targets USP8 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag27104

Product name: Recombinant human USP8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 15-253 aa of BC110590

Sequence: SSLKDLNKKTEVKPEKISTKSYVHSALKIFKTAEECRLDRDEERAYVLYMKYVTVYNLIKKRPDFKQQQDYFHSILGPGNIKKAVEEAERLSESLKLRYEEAEVRKKLEEKDRQEEAQRLQQKRQETGREDGGTLAKGSLENVLDSKDKTQKSNGEKNEKCETKEKGAITAKELYTMMTDKNISLIIMDARRMQDYQDSCILHSLSVPEEAISPGVTASWIEAHLPDDSKDTWKKRGNV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 8
别名 1E12E3, EC:3.4.19.12, hUBPy, HumORF8, KIAA0055
计算分子量 1118 aa, 128 kDa
观测分子量 128 kDa
GenBank蛋白编号BC110590
基因名称 USP8
Gene ID (NCBI) 9101
RRIDAB_2934535
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
激发激光Blue laser (488 nm)
形式Liquid
纯化方式Protein G purification
UNIPROT IDP40818
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP8 is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 USP8 antibody CL488-67321Download protocol
IF protocol for CL Plus 488 USP8 antibody CL488-67321Download protocol
Standard Protocols
Click here to view our Standard Protocols
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