USP8 Monoclonal antibody
USP8 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse and More (1)
Applications
FC, IF/ICC, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
1E12E3
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, HEK-293 cells, LNcaP cells, Jurkat cells, K-562 cells, 4T1 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67321-1-Ig targets USP8 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, CoIP |
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human |
| Immunogen | USP8 fusion protein Ag27104 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ubiquitin specific peptidase 8 |
| Synonyms | Deubiquitinating enzyme 8, FLJ34456, hUBPy, HumORF8, KIAA0055, Ubiquitin isopeptidase Y, ubiquitin specific peptidase 8, Ubiquitin thiolesterase 8, UBPY, USP8 |
| Calculated Molecular Weight | 1118 aa, 128 kDa |
| Observed Molecular Weight | 128 kDa |
| GenBank Accession Number | BC110590 |
| Gene Symbol | USP8 |
| Gene ID (NCBI) | 9101 |
| RRID | AB_2882581 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P40818 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP8 antibody 67321-1-Ig | Download protocol |
| IHC protocol for USP8 antibody 67321-1-Ig | Download protocol |
| IF protocol for USP8 antibody 67321-1-Ig | Download protocol |
| FC protocol for USP8 antibody 67321-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization | ||
J Hum Genet A heterozygous germline deletion within USP8 causes severe neurodevelopmental delay with multiorgan abnormalities | ||
J Adv Res The ubiquitin-specific protease 8 antagonizes melatonin-induced endocytic degradation of MT(1) receptor to promote lung adenocarcinoma growth
| ||
Adv Sci (Weinh) Targeting USP8 Inhibits O-GlcNAcylation of SLC7A11 to Promote Ferroptosis of Hepatocellular Carcinoma via Stabilization of OGT |
