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验证数据展示

  • WB analysis using 67321-1-Ig (same clone as 67321-1-PBS)

    WB analysis using 67321-1-Ig (same clone as 67321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67321-1-Ig (same clone as 67321-1-PBS)

    IHC staining of human breast cancer using 67321-1-Ig (same clone as 67321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67321-1-Ig (USP8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with USP8 antibody included
  • IHC staining of human breast cancer using 67321-1-Ig (same clone as 67321-1-PBS)

    IHC staining of human breast cancer using 67321-1-Ig (same clone as 67321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67321-1-Ig (USP8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with USP8 antibody included
  • FC experiment of Hela using 67321-1-Ig (same clone as 67321-1-PBS)

    FC experiment of Hela using 67321-1-Ig (same clone as 67321-1-PBS)

    1X10^6 Hela cells were intracellularly stained with 0.4 ug Anti-Human USP8 (67321-1-Ig, Clone:1E12E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 67321-1-Ig (same clone as 67321-1-PBS)

    WB analysis of NIH/3T3 using 67321-1-Ig (same clone as 67321-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67321-1-Ig (same clone as 67321-1-PBS)

    IF Staining of HeLa using 67321-1-Ig (same clone as 67321-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP8 antibody (67321-1-Ig, Clone: 1E12E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67321-1-Ig (same clone as 67321-1-PBS)

    FC experiment of HeLa using 67321-1-Ig (same clone as 67321-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human USP8 (67321-1-Ig, Clone:1E12E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67321-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

USP8 Monoclonal antibody, PBS Only

USP8 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67321-1-PBS

产品说明书

CloneNo. 1E12E3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

1E12E3, EC:3.4.19.12, hUBPy, HumORF8, KIAA0055

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy USP8 Ready-To-Use IHC Kit. USP8 primary antibody included.

产品信息

67321-1-PBS targets USP8 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag27104

Product name: Recombinant human USP8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 15-253 aa of BC110590

Sequence: SSLKDLNKKTEVKPEKISTKSYVHSALKIFKTAEECRLDRDEERAYVLYMKYVTVYNLIKKRPDFKQQQDYFHSILGPGNIKKAVEEAERLSESLKLRYEEAEVRKKLEEKDRQEEAQRLQQKRQETGREDGGTLAKGSLENVLDSKDKTQKSNGEKNEKCETKEKGAITAKELYTMMTDKNISLIIMDARRMQDYQDSCILHSLSVPEEAISPGVTASWIEAHLPDDSKDTWKKRGNV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 8
别名 1E12E3, EC:3.4.19.12, hUBPy, HumORF8, KIAA0055
计算分子量 1118 aa, 128 kDa
观测分子量 128 kDa
GenBank蛋白编号BC110590
基因名称 USP8
Gene ID (NCBI) 9101
RRIDAB_2882581
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP40818
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP8 is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage.

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