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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated UCHL1/PGP9.5 Monoclonal antibody

UCHL1/PGP9.5 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1C9E11

Cat No : CL647-66230

Print datasheet

Synonyms

Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1

验证数据展示

  • IF Staining of SH-SY5Y using CL647-66230

    IF Staining of SH-SY5Y using CL647-66230

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 647 UCHL1/PGP9.5 antibody (CL647-66230, Clone: 1C9E11 ) at dilution of 1:200.

  • FC experiment of SH-SY5Y using CL647-66230

    FC experiment of SH-SY5Y using CL647-66230

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human UCHL1/PGP9.5 (CL647-66230, Clone:1C9E11) (red), or 0.2 ug APC-65128 ; APC Mouse IgG2b Isotype Control (APC-65128, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 66230-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66230 targets UCHL1/PGP9.5 in IF, FC (Intra) applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, pig, rat
Immunogen UCHL1/PGP9.5 fusion protein Ag6547 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Synonyms Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC000332
Gene Symbol UCHL1
Gene ID (NCBI) 7345
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09936
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin C-terminal hydrolase L1 (UCHL1) was originally identified as a neuronal protein that accounts for nearly 2% of total brain proteins. UCHL1 activity protects neurons from hypoxic injury, and binding of stroke-induced reactive lipid species to the cysteine 152 (C152) of UCHL1 unfolds the protein and disrupts its function. Reduced hydrolytic activity of mutant UCHL1 is implicated in the pathophysiologic process of Parkinson's and Alzheimer's disease due to abnormal neurotoxic protein aggregation. (PMID: 31356902, PMID: 30760601)

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 UCHL1/PGP9.5 antibody CL647-66230Download protocol
FC protocol for CL Plus 647 UCHL1/PGP9.5 antibody CL647-66230Download protocol
Standard Protocols
Click here to view our Standard Protocols