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UCHL1/PGP9.5 Monoclonal antibody
UCHL1/PGP9.5 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig, rat
Applications
FC, IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1C9E11
验证数据展示
经过测试的应用
Positive WB detected in | Neuro-2a cells, Y79 cells, PC-12 cells, Pig brain tissue, Pig cerebellum tissue, Rat brain tissue, Rat cerebellum tissue, Mouse brain tissue, Mouse cerebellum tissue |
Positive IHC detected in | mouse cerebellum tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | SH-SY5Y cells |
Positive FC detected in | Y79 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66230-1-Ig targets UCHL1/PGP9.5 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, pig, rat |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | UCHL1/PGP9.5 fusion protein Ag6547 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
Synonyms | Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1, UCH-L1, UCHL1/PGP9.5 |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 27 kDa |
GenBank Accession Number | BC000332 |
Gene Symbol | UCHL1 |
Gene ID (NCBI) | 7345 |
RRID | AB_2881621 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P09936 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for UCHL1/PGP9.5 antibody 66230-1-Ig | Download protocol |
IHC protocol for UCHL1/PGP9.5 antibody 66230-1-Ig | Download protocol |
IF protocol for UCHL1/PGP9.5 antibody 66230-1-Ig | Download protocol |
FC protocol for UCHL1/PGP9.5 antibody 66230-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Theranostics UCH-L1-mediated Down-regulation of Estrogen Receptor α Contributes to Insensitivity to Endocrine Therapy for Breast Cancer.
| ||
Toxicol Appl Pharmacol Ursolic acid alleviates paclitaxel-induced peripheral neuropathy through PPARγ activation | ||
Dig Dis Sci P2Y1, P2Y2, and TRPV1 Receptors Are Increased in Diarrhea-Predominant Irritable Bowel Syndrome and P2Y2 Correlates with Abdominal Pain. | ||
Reprod Domest Anim Isolation and in vitro expansion of porcine spermatogonial stem cells. | ||
ORL J Otorhinolaryngol Relat Spec Role of the Ubiquitin C-Terminal Hydrolase L1-Modulated Ubiquitin Proteasome System in Auditory Cortex Senescence. | ||
Discov Oncol Oxaliplatin-induced peripheral neuropathy can be minimized by pressurized regional intravascular delivery in an orthotopic murine pancreatic cancer model. |