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CoraLite® Plus 488-conjugated UCHL1/PGP9.5 Monoclonal antibody

UCHL1/PGP9.5 Monoclonal Antibody for FC (Intra), IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

FC (Intra), IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C9E11

Cat No : CL488-66230

Print datasheet

Synonyms

Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1, UCHL1/PGP9.5

验证数据展示

  • IF Staining of mouse brain using CL488-66230

    IF Staining of mouse brain using CL488-66230

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 UCHL1/PGP9.5 antibody (CL488-66230, Clone: 1C9E11 ) at dilution of 1:200.

  • FC experiment of Y79 using CL488-66230

    FC experiment of Y79 using CL488-66230

    1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human UCHL1/PGP9.5 (CL488-66230, Clone:1C9E11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inmouse brain tissue
Positive FC detected inY79 cells
For other applications, we recommend the unconjugated version of this antibody, 66230-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66230 targets UCHL1/PGP9.5 in IF, FC (Intra) applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity human, mouse, pig, rat
Immunogen UCHL1/PGP9.5 fusion protein Ag6547 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Synonyms Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1, UCHL1/PGP9.5
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC000332
Gene Symbol UCHL1
Gene ID (NCBI) 7345
RRIDAB_2883290
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09936
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 UCHL1/PGP9.5 antibody CL488-66230Download protocol
FC protocol for CL Plus 488 UCHL1/PGP9.5 antibody CL488-66230Download protocol
Standard Protocols
Click here to view our Standard Protocols