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验证数据展示

  • WB analysis using CL488-67198

    WB analysis using CL488-67198

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67198 (UBE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 488-conjugated UBE1 Monoclonal antibody

UBE1 Monoclonal Antibody for WB
Cat No. CL488-67198

产品说明书

CloneNo. 1H7E8

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB

别名

A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy UBA1 Ready-To-Use IHC Kit. UBA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67198-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67198 targets UBE1 in WB applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB Application Description
经测试反应性 Human, mouse, rat
免疫原 UBE1 fusion protein Ag8920 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin-like modifier activating enzyme 1
别名 A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1
计算分子量 1058 aa, 118 kDa
观测分子量 114-118 kDa
GenBank蛋白编号BC013041
基因名称 UBA1
Gene ID (NCBI) 7317
RRIDAB_3084324
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP22314
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 UBE1 antibody CL488-67198Download protocol
Standard Protocols
Click here to view our Standard Protocols