验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HeLa cell, HEK-293 cells, Jurkat cells, HL-60 cells, K-562 cells, HSC-T6 cells, RAW 264.7 cells, NIH/3T3 cells |
| Positive IHC detected in | human colon cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy UBA1 Ready-To-Use IHC Kit. UBA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:30000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
67198-1-Ig targets UBE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag8920 Product name: Recombinant human UBE1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 711-1058 aa of BC013041 Sequence: CHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin-like modifier activating enzyme 1 |
| 别名 | A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1 |
| 计算分子量 | 1058 aa, 118 kDa |
| 观测分子量 | 114-118 kDa |
| GenBank蛋白编号 | BC013041 |
| 基因名称 | UBA1 |
| Gene ID (NCBI) | 7317 |
| RRID | AB_2882491 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P22314 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for UBE1 antibody 67198-1-Ig | Download protocol |
| IHC protocol for UBE1 antibody 67198-1-Ig | Download protocol |
| WB protocol for UBE1 antibody 67198-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
