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验证数据展示

  • WB analysis using 67198-1-Ig (same clone as 67198-1-PBS)

    WB analysis using 67198-1-Ig (same clone as 67198-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67198-1-Ig (UBE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67198-1-Ig (same clone as 67198-1-PBS)

    IHC staining of human colon cancer using 67198-1-Ig (same clone as 67198-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBA1 antibody included
  • IHC staining of human colon cancer using 67198-1-Ig (same clone as 67198-1-PBS)

    IHC staining of human colon cancer using 67198-1-Ig (same clone as 67198-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBA1 antibody included
  • Cytometric bead array sample test of MP51632-1

    Cytometric bead array sample test of MP51632-1

    Cytometric bead array sample test of MP51632-1, UBE1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67198-2-PBS. Detection antibody: 67198-1-PBS.

  • IF Staining of HeLa using 67198-1-Ig (same clone as 67198-1-PBS)

    IF Staining of HeLa using 67198-1-Ig (same clone as 67198-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE1 antibody (67198-1-Ig, Clone: 1H7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51632-1

    Cytometric bead array standard curve of MP51632-1

    Cytometric bead array standard curve of MP51632-1, UBE1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67198-2-PBS. Detection antibody: 67198-1-PBS. Standard:Ag8920. Range: 0.781-100 ng/mL.

UBE1 Monoclonal antibody, PBS Only (Detector)

UBE1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67198-1-PBS

产品说明书

CloneNo. 1H7E8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

UBA1, 1H7E8, A1S9, A1S9T, A1ST

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy UBA1 Ready-To-Use IHC Kit. UBA1 primary antibody included.

产品信息

67198-1-PBS targets UBE1 as part of a matched antibody pair:

MP51632-1: 67198-2-PBS capture and 67198-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8920

Product name: Recombinant human UBE1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 711-1058 aa of BC013041

Sequence: CHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin-like modifier activating enzyme 1
别名 UBA1, 1H7E8, A1S9, A1S9T, A1ST
计算分子量 1058 aa, 118 kDa
观测分子量 114-118 kDa
GenBank蛋白编号BC013041
基因名称 UBA1
Gene ID (NCBI) 7317
RRIDAB_2882491
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP22314
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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