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验证数据展示

  • FC experiment of A549 using CL488-18236

    FC experiment of A549 using CL488-18236

    1x10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated TRPC6 Polyclonal antibody (CL488-18236)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of human placenta using CL488-18236

    IF Staining of human placenta using CL488-18236

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 TRPC6 antibody (CL488-18236) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated TRPC6 Polyclonal antibody

TRPC6 Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-18236

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P, FC (Intra)

别名

TRPC 6, TRP-6, TRP6, TRP 6, Transient receptor protein 6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman placenta tissue
Positive FC (Intra) detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 18236-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-18236 targets TRPC6 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 TRPC6 fusion protein Ag12976 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transient receptor potential cation channel, subfamily C, member 6
别名 TRPC 6, TRP-6, TRP6, TRP 6, Transient receptor protein 6
计算分子量 931 aa, 106 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC093658
基因名称 TRPC6
Gene ID (NCBI) 7225
RRIDAB_3084017
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y210
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transient receptor potential canonical (TRPC) channels mediate the influx of different types of cations through the cell membrane and are involved in many functions of the organism (PMID: 28508319). The TRPC subfamily consists of seven members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, TRPC7 (PMID: 32872338). TRPC6 is a Ca(2+)-permeable non-selective cation channel expressed in most human tissues. TRPC6 has been considered as potential therapeutic targets for malignant glioma treatment (PMID: 35489424).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TRPC6 antibody CL488-18236Download protocol
FC protocol for CL Plus 488 TRPC6 antibody CL488-18236Download protocol
Standard Protocols
Click here to view our Standard Protocols