验证数据展示

WB analysis of mouse brain using 18236-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500.
WB analysis using 18236-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 18236-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 18236-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP experiment of mouse lung using 18236-1-AP
IP result of anti-TRPC6 (IP:18236-1-AP, 4ug; Detection:18236-1-AP 1:300) with mouse lung tissue lysate 3600ug.
FC experiment of A549 using 18236-1-AP
1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human TRPC6 (18236-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
IF Staining of human placenta using 18236-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using TRPC6 antibody (18236-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 18236-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRPC6 antibody (18236-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO验证

TRPC6 Polyclonal antibody

TRPC6 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 18236-1-AP

产品说明书

引用文献(17)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (1)

别名

FSGS2, Transient receptor protein 6, TRP 6, TRP6, TRP-6

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (17)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse lung tissue, COLO 320 cells, mouse brain tissue
Positive IP detected in	mouse lung tissue
Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human placenta tissue
Positive IF/ICC detected in	A549 cells
Positive FC (Intra) detected in	A549 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 11 publications below
IHC	See 1 publications below
IF	See 9 publications below
IP	See 1 publications below

产品信息

18236-1-AP targets TRPC6 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag12976 Product name: Recombinant human TRPC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-405 aa of BC093658 Sequence: MSQSPAFGPRRGSSPRGAAGAAARRNESQDYLLMDSELGEDGCPQAPLPCYGYYPCFRGSDNRLAHRRQTVLREKGRRLANRGPAYMFSDRSTSLSIEEERFLDAAEYGNIPVVRKMLEECHSLNVNCVDYMGQNALQLAVANEHLEITELLLKKENLSRVGDALLLAISKGYVRIVEAILSHPAFAEGKRLATSPSQSELQQDDFYAYDEDGTRFSHDVTPIILAAHCQEYEIVHTLLRKGARIERPHDYFCKCNDCNQKQKHDSFSHSRSRINAYKGLASPAYLSLSSEDPVMTALELSNELAVLANIEKEFKNDYKKLSMQCKDFVVGLLDLCRNTEEVEAILNGDVETLQSGDHGRPNLSRLKLAIKYEVKKFVAHPNCQQQLLSIWYENLSGLRQQTMAV 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	transient receptor potential cation channel, subfamily C, member 6
别名	FSGS2, Transient receptor protein 6, TRP 6, TRP6, TRP-6
计算分子量	931 aa, 106 kDa
观测分子量	100-110 kDa
GenBank蛋白编号	BC093658
基因名称	TRPC6
Gene ID (NCBI)	7225
RRID	AB_10859822
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9Y210
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Transient receptor potential canonical (TRPC) channels mediate the influx of different types of cations through the cell membrane and are involved in many functions of the organism (PMID: 28508319). The TRPC subfamily consists of seven members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, TRPC7 (PMID: 32872338). TRPC6 is a Ca(2+)-permeable non-selective cation channel expressed in most human tissues. TRPC6 has been considered as potential therapeutic targets for malignant glioma treatment (PMID: 35489424).

实验方案

Product Specific Protocols
IF protocol for TRPC6 antibody 18236-1-AP	Download protocol
IHC protocol for TRPC6 antibody 18236-1-AP	Download protocol
IP protocol for TRPC6 antibody 18236-1-AP	Download protocol
WB protocol for TRPC6 antibody 18236-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human		Cell Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis. Authors - Wei Rao View Article
mouse	WB	J Hazard Mater Epigenetic reprogramming of HDAC2 in CA1 excitatory neurons determines Pb-induced non-spatial memory deficits Authors - Mengmeng Wang View Article
mouse	WB,IF	Biomaterials Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation. Authors - Meitian Wang View Article
human	IF	Proc Natl Acad Sci U S A TRPC6 channel translocation into phagosomal membrane augments phagosomal function. Authors - Vladimir Riazanski KO Validated View Article
mouse	WB	Phytomedicine Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro Authors - Zhongzhu Ai View Article
mouse	WB	Br J Pharmacol Catalpol Alleviates Adriamycin-Induced Nephropathy by Activating the SIRT1 Signalling Pathway In Vivo and In Vitro. Authors - Jiangnan Zhang View Article

验证数据展示

WB analysis of mouse brain using 18236-1-AP

WB analysis using 18236-1-AP

IHC staining of human placenta using 18236-1-AP

IHC staining of human placenta using 18236-1-AP

IP experiment of mouse lung using 18236-1-AP

FC experiment of A549 using 18236-1-AP

IF Staining of human placenta using 18236-1-AP

IF Staining of A549 using 18236-1-AP

TRPC6 Polyclonal antibody

TRPC6 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis.

Epigenetic reprogramming of HDAC2 in CA1 excitatory neurons determines Pb-induced non-spatial memory deficits

Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation.

TRPC6 channel translocation into phagosomal membrane augments phagosomal function.

Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro

Catalpol Alleviates Adriamycin-Induced Nephropathy by Activating the SIRT1 Signalling Pathway In Vivo and In Vitro.