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验证数据展示

  • WB analysis using 85150-4-RR

    WB analysis using 85150-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85150-4-RR (CRTC2,TORC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 85150-4-RR

    FC experiment of HeLa using 85150-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TORC2 Recombinant antibody (85150-4-RR, Clone:242808B6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 85150-4-RR

    Affinity and Kinetic Characterization of 85150-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85150-4-RR against Human TORC2 were performed. The affinity constant is 0.422 nM.

TORC2 Recombinant antibody

TORC2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 85150-4-RR

产品说明书

CloneNo. 242808B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

CRTC2, CRTC2,TORC2, 242808B6, CREB-regulated transcription coactivator 2, TORC 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, Raji cells, A-673 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CRTC2 Ready-To-Use IHC Kit. CRTC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85150-4-RR targets TORC2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 TORC2 fusion protein Ag3167 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CREB regulated transcription coactivator 2
别名 CRTC2, CRTC2,TORC2, 242808B6, CREB-regulated transcription coactivator 2, TORC 2
计算分子量 693 aa, 73 kDa
观测分子量80 kDa
GenBank蛋白编号BC053562
基因名称 TORC2
Gene ID (NCBI) 200186
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ53ET0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .

实验方案

Product Specific Protocols
WB protocol for TORC2 antibody 85150-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols