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Host
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Species Reactivity
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验证数据展示

  • WB analysis using 85150-4-RR (same clone as 85150-4-PBS)

    WB analysis using 85150-4-RR (same clone as 85150-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85150-4-RR (CRTC2,TORC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85150-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 85150-4-RR (same clone as 85150-4-PBS)

    FC experiment of HeLa using 85150-4-RR (same clone as 85150-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TORC2 Recombinant antibody (85150-4-RR, Clone:242808B6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85150-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85150-4-RR

    Affinity and Kinetic Characterization of 85150-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85150-4-RR against Human TORC2 were performed. The affinity constant is 0.422 nM.

TORC2 Recombinant antibody, PBS Only

TORC2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 85150-4-PBS

产品说明书

CloneNo. 242808B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

CRTC2, CRTC2,TORC2, 242808B6, CREB-regulated transcription coactivator 2, TORC 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CRTC2 Ready-To-Use IHC Kit. CRTC2 primary antibody included.

产品信息

85150-4-PBS targets TORC2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 TORC2 fusion protein Ag3167 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CREB regulated transcription coactivator 2
别名 CRTC2, CRTC2,TORC2, 242808B6, CREB-regulated transcription coactivator 2, TORC 2
计算分子量 693 aa, 73 kDa
观测分子量80 kDa
GenBank蛋白编号BC053562
基因名称 TORC2
Gene ID (NCBI) 200186
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ53ET0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .