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TNIK Monoclonal antibody

TNIK Monoclonal Antibody for WB,IHC,IF/ICC,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB,IHC,IF/ICC,ELISA

Conjugate

Unconjugated

CloneNo.

1E9H3

Cat No : 67948-1-Ig

Print datasheet

Synonyms

KIAA0551, TNIK

验证数据展示

  • WB analysis of K-562 using 67948-1-Ig

    WB analysis of K-562 using 67948-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of human peripheral blood platelets using 67948-1-Ig

    WB analysis of human peripheral blood platelets using 67948-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 67948-1-Ig

    IHC staining of human kidney using 67948-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 67948-1-Ig

    IHC staining of human kidney using 67948-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 67948-1-Ig

    IF Staining of A431 using 67948-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TNIK antibody (67948-1-Ig, Clone: 1E9H3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inhuman peripheral blood platelets, K-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67948-1-Ig targets TNIK in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB,IHC,IF/ICC,ELISA
Tested Reactivity Human
Immunogen TNIK fusion protein Ag30710 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name TRAF2 and NCK interacting kinase
Synonyms KIAA0551, TNIK
Calculated Molecular Weight 155 kDa
Observed Molecular Weight155 kDa
GenBank Accession NumberBC055427
Gene Symbol TNIK
Gene ID (NCBI) 23043
RRIDAB_2918700
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TNIK antibody 67948-1-IgDownload protocol
IHC protocol for TNIK antibody 67948-1-IgDownload protocol
IF protocol for TNIK antibody 67948-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols