验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IP | See 1 publications below |
| CoIP | See 1 publications below |
产品信息
65208-1-Ig targets IgG2a Isotype Control in FC, IP, CoIP applications and shows reactivity with samples.
| 经测试应用 | FC Application Description |
| 文献引用应用 | IP, CoIP |
| 经测试反应性 | |
| 免疫原 |
N/A 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | IgG2a Isotype Control |
| 别名 | IgG2a, IgG2a Isotype Control, Mouse IgG2a Isotype Control |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2918470 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
The C1.18.4 immunoglobulin is useful as an isotype-matched control. The C.1.18.4 immunolglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for IgG2a Isotype Control antibody 65208-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification | ||
J Nutr Sci Vitaminol (Tokyo) Cooperation of Cysteine, Ascorbic Acid, Pyridoxine, and α-Lipoic Acid in Antioxidant Effects on Human Keratinocytes and 3D Human Skin Models | ||
Mol Med Progesterone (P4) ameliorates cigarette smoke-induced chronic obstructive pulmonary disease (COPD) |
