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验证数据展示

  • WB analysis of K-562 using 67948-1-Ig (same clone as 67948-1-PBS)

    WB analysis of K-562 using 67948-1-Ig (same clone as 67948-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • WB analysis of human peripheral blood platelets using 67948-1-Ig (same clone as 67948-1-PBS)

    WB analysis of human peripheral blood platelets using 67948-1-Ig (same clone as 67948-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67948-1-Ig (same clone as 67948-1-PBS)

    IHC staining of human kidney using 67948-1-Ig (same clone as 67948-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67948-1-Ig (same clone as 67948-1-PBS)

    IHC staining of human kidney using 67948-1-Ig (same clone as 67948-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 67948-1-Ig (same clone as 67948-1-PBS)

    IF Staining of A431 using 67948-1-Ig (same clone as 67948-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TNIK antibody (67948-1-Ig, Clone: 1E9H3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 67948-1-Ig (same clone as 67948-1-PBS)

    FC experiment of A431 using 67948-1-Ig (same clone as 67948-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TNIK (67948-1-Ig, Clone:1E9H3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

TNIK Monoclonal antibody, PBS Only

TNIK Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67948-1-PBS

产品说明书

CloneNo. 1E9H3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

1E9H3, EC:2.7.11.1, KIAA0551, TRAF2 and NCK-interacting protein kinase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67948-1-PBS targets TNIK in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 TNIK fusion protein Ag30710 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 TRAF2 and NCK interacting kinase
别名 1E9H3, EC:2.7.11.1, KIAA0551, TRAF2 and NCK-interacting protein kinase
计算分子量 155 kDa
观测分子量155~180 kDa
GenBank蛋白编号BC055427
基因名称 TNIK
Gene ID (NCBI) 23043
RRIDAB_2918700
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UKE5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Traf2- and Nck-interacting kinase (TNIK) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension (PMID: 24566388). TNIK is an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462).Western blot analysis detected TNIK at an apparent molecular mass of 150~180 kDa as cited in literature (PMID: 24566388).