验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | A431 cells |
| Positive FC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67948 targets TNIK in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag30710 Product name: Recombinant human TNIK protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 157-538 aa of BC055427 Sequence: PRVYQTSPTDEDEEDEESSATALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | TRAF2 and NCK interacting kinase |
| 别名 | TNIK, TRAF2 and NCK-interacting protein kinase, 1E9H3, EC:2.7.11.1, KIAA0551 |
| 计算分子量 | 155 kDa |
| GenBank蛋白编号 | BC055427 |
| 基因名称 | TNIK |
| Gene ID (NCBI) | 23043 |
| RRID | AB_3084791 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UKE5 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Traf2- and Nck-interacting kinase (TNIK) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension (PMID: 24566388). TNIK is an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 TNIK antibody CL594-67948 | Download protocol |
| IF protocol for CL594 TNIK antibody CL594-67948 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
