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验证数据展示

  • IF Staining of HeLa using CL488-13359

    IF Staining of HeLa using CL488-13359

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TDRD3 antibody (CL488-13359) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HEK-293 using CL488-13359

    FC experiment of HEK-293 using CL488-13359

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Tdrd3 Polyclonal Antibody (CL488-13359)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated TDRD3 Polyclonal antibody

TDRD3 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-13359

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Tudor domain-containing protein 3, tudor domain containing 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TDRD3 Ready-To-Use IHC Kit. TDRD3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13359-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13359 targets TDRD3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 TDRD3 fusion protein Ag4174 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tudor domain containing 3
别名 Tudor domain-containing protein 3, tudor domain containing 3
计算分子量 651 aa, 73 kDa
观测分子量 73 kDa, 83 kDa
GenBank蛋白编号BC030514
基因名称 TDRD3
Gene ID (NCBI) 81550
RRIDAB_3672562
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H7E2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TDRD3 (Tudor domain-containing protein 3) contains tudor domain which is an approximately 60-amino acid structure motif. TDRD3 functions as a scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. TDRD3 is a transcriptional coactivator that promotes transcription by binding methylarginine marks on histone tails. TDRD3 also possesses an oligosaccharide/nucleotide binding fold and an ubiquitin associated domain capable of binding tetra-ubiquitin, and it is reported to associate with polyribosomes in HeLa cells.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TDRD3 antibody CL488-13359Download protocol
FC protocol for CL Plus 488 TDRD3 antibody CL488-13359Download protocol
Standard Protocols
Click here to view our Standard Protocols