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验证数据展示

  • IHC staining of mouse brain using 13359-1-AP

    IHC staining of mouse brain using 13359-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13359-1-AP (TDRD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TDRD3 antibody included
  • IHC staining of mouse brain using 13359-1-AP

    IHC staining of mouse brain using 13359-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13359-1-AP (TDRD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TDRD3 antibody included
  • WB analysis of HEK-293 using 13359-1-AP

    WB analysis of HEK-293 using 13359-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13359-1-AP (TDRD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 13359-1-AP

    WB analysis of HEK-293 using 13359-1-AP

    WB result of TDRD3 antibody (13359-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDRD3 transfected HEK-293 cells.

  • IP experiment of HeLa using 13359-1-AP

    IP experiment of HeLa using 13359-1-AP

    IP result of anti-TDRD3 (IP:13359-1-AP, 3ug; Detection:13359-1-AP 1:1000) with HeLa cells lysate 3800ug.

  • IF Staining of HeLa using 13359-1-AP

    IF Staining of HeLa using 13359-1-AP

    Immunofluorescent analysis of HeLa cells using 13359-1-AP (TDRD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • Featured Product
  • KD/KO验证

TDRD3 Polyclonal antibody

TDRD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13359-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

Tudor domain-containing protein 3, tudor domain containing 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TDRD3 Ready-To-Use IHC Kit. TDRD3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13359-1-AP targets TDRD3 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, chIP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag4174

Product name: Recombinant human TDRD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 301-651 aa of BC030514

Sequence: YRSSNTEQNGVKDNNHLRHPPRNDTRQPRNEKPPRFQRDSQNSKSVLEGSGLPRNRGSERPSTSSVSEVWAEDRIKCDRPYSRYDRTKDTSYPLGSQHSDGAFKKRDNSMQSRSGKGPSFAEAKENPLPQGSVDYNNQKRGKRESQTSIPDYFYDRKSQTINNEAFSGIKIEKHFNVNTDYQNPVRSNSFIGVPNGEVEMPLKGRRIGPIKPAGPVTAVPCDDKIFYNSGPKRRSGPIKPEKILESSIPMEYAKMWKPGDECFALYWEDNKFYRAEVEALHSSGMTAVVKFIDYGNYEEVLLSNIKPIQTEAWEEEGTYDQTLEFRRGGDGQPRRSTRPTQQFYQPPRARN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tudor domain containing 3
别名 Tudor domain-containing protein 3, tudor domain containing 3
计算分子量 651 aa, 73 kDa
观测分子量 73 kDa, 83 kDa
GenBank蛋白编号BC030514
基因名称 TDRD3
Gene ID (NCBI) 81550
RRIDAB_2202810
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H7E2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TDRD3 (Tudor domain-containing protein 3) contains tudor domain which is an approximately 60-amino acid structure motif. TDRD3 functions as a scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. TDRD3 is a transcriptional coactivator that promotes transcription by binding methylarginine marks on histone tails. TDRD3 also possesses an oligosaccharide/nucleotide binding fold and an ubiquitin associated domain capable of binding tetra-ubiquitin, and it is reported to associate with polyribosomes in HeLa cells.

实验方案

Product Specific Protocols
WB protocol for TDRD3 antibody 13359-1-APDownload protocol
IHC protocol for TDRD3 antibody 13359-1-APDownload protocol
IF protocol for TDRD3 antibody 13359-1-APDownload protocol
IP protocol for TDRD3 antibody 13359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanchIP

Theranostics

A hypermethylation strategy utilized by enhancer-bound CARM1 to promote estrogen receptor α-dependent transcriptional activation and breast carcinogenesis.

Authors - Bing-Ling Peng
View Article
humanWB

Int J Biol Macromol

PRMT1 and TDRD3 promote stress granule assembly by rebuilding the protein-RNA interaction network

Authors - Mengtong Qin
View Article