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验证数据展示

  • WB analysis using 86138-1-RR

    WB analysis using 86138-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86138-1-RR (SPINT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 86138-1-RR

    WB analysis of MCF-7 using 86138-1-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 86138-1-RR (SPINT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86138-1-RR

    WB analysis using 86138-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86138-1-RR (SPINT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

SPINT1/HAI-1 Recombinant antibody

SPINT1/HAI-1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86138-1-RR

产品说明书

CloneNo. 250560F1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

HAI 1, HAI-1, SPINT1, HAI, HAI1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inT-47D cells, MCF-7 cells, mouse small intestine tissue, mouse colon tissue, Daudi cells, human placenta

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86138-1-RR targets SPINT1/HAI-1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 SPINT1/HAI-1 fusion protein Eg3131 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 serine peptidase inhibitor, Kunitz type 1
别名 HAI 1, HAI-1, SPINT1, HAI, HAI1
计算分子量58 kDa
观测分子量58-70 kDa
GenBank蛋白编号NM_181642.2
基因名称 HAI-1
Gene ID (NCBI) 6692
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43278-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is also named as SPINT1 and Kunitz-type protease inhibitor 1. HAI-1 is critical to control matriptase proteolytic activity (PMID: 25310042). HAI-1 inhibits matriptase activity through forming complex with activated matriptase (PMID: 30370662). matriptase, HAI‐1 also binds to and inhibits a variety of serine proteases including of hepsin, plasmin, prostasin, TMPRSS13 and HAT. HAI-1 as a cell-autonomous tumor suppressor that negatively regulates the HGF pathway (PMID: 25925948). HAI-1 is inhibitor of HGFAC (PMID: 9045658). HAI-1 inhibits serine protease activity of ST14/matriptase in vitro (PMID: 28710277). HAI-1 inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain (PMID: 20977675). HAI-1 expression is reduced in many cancer types, which is implicated in the progression of cancer and is associated with a worse prognosis in cancer patients (PMID: 30370662).

实验方案

Product Specific Protocols
WB protocol for SPINT1/HAI-1 antibody 86138-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols