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验证数据展示

  • WB analysis of Daudi using 66618-2-Ig (same clone as 66618-2-PBS)

    WB analysis of Daudi using 66618-2-Ig (same clone as 66618-2-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 66618-2-Ig (same clone as 66618-2-PBS)

    IHC staining of mouse spleen using 66618-2-Ig (same clone as 66618-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPI1 antibody included
  • IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPI1 antibody included
  • IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPI1 antibody included
  • IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:2000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPI1 antibody included

SPI1 Monoclonal antibody, PBS Only

SPI1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66618-2-PBS

产品说明书

CloneNo. 2H3D3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

2H3D3, 31 kDa transforming protein, 31 kDa-transforming protein, OF, PU.1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SPI1 Ready-To-Use IHC Kit. SPI1 primary antibody included.

产品信息

66618-2-PBS targets SPI1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 spleen focus forming virus (SFFV) proviral integration oncogene spi1
别名 2H3D3, 31 kDa transforming protein, 31 kDa-transforming protein, OF, PU.1
计算分子量 31 kDa
观测分子量 32-38 kDa
GenBank蛋白编号NM_003120
基因名称 SPI1
Gene ID (NCBI) 6688
ENSEMBL Gene IDENSG00000066336
RRIDAB_2881978
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP17947
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV.

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