验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, Raji cells, RAW 264.7 cells, rat spleen tissue |
| Positive IHC detected in | Human tonsillitis tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SPI1 Ready-To-Use IHC Kit. SPI1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66618-2-Ig targets SPI1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | spleen focus forming virus (SFFV) proviral integration oncogene spi1 |
| 别名 | SFPI1, PU.1, OF, 31 kDa-transforming protein, 31 kDa transforming protein |
| 计算分子量 | 31 kDa |
| 观测分子量 | 32-38 kDa |
| GenBank蛋白编号 | NM_003120 |
| 基因名称 | SPI1 |
| Gene ID (NCBI) | 6688 |
| ENSEMBL Gene ID | ENSG00000066336 |
| RRID | AB_2881978 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P17947 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPI1 antibody 66618-2-Ig | Download protocol |
| IHC protocol for SPI1 antibody 66618-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Immunother Cancer SPI1+CD68+ macrophages as a biomarker for gastric cancer metastasis: a rationale for combined antiangiogenic and immunotherapy strategies | ||
Discov Oncol SPI1 is a prognostic biomarker of immune infiltration and immunotherapy efficacy in clear cell renal cell carcinoma | ||
Cancers (Basel) ACAP1 Deficiency Predicts Inferior Immunotherapy Response in Solid Tumors | ||
Exp Cell Res IRF1 suppresses colon cancer proliferation by reducing SPI1-mediated transcriptional activation of GPX4 and promoting ferroptosis | ||
Biomedicines Exploring the Role of Neutrophil-Related Genes in Osteosarcoma via an Integrative Analysis of Single-Cell and Bulk Transcriptome |
