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验证数据展示

  • WB analysis using 86015-1-RR

    WB analysis using 86015-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86015-1-RR (SMARCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 86015-1-RR

    IF Staining of Jurkat using 86015-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SMARCC1 antibody (86015-1-RR, Clone: 250455A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86015-1-RR

    Affinity and Kinetic Characterization of 86015-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86015-1-RR against Human SMARCC1 were performed. The affinity constant is 0.393 nM.

SMARCC1 Recombinant antibody

SMARCC1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86015-1-RR

产品说明书

CloneNo. 250455A1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

BAF155, BRG1-associated factor 155, CRACC1, Rsc8, SRG3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNeuro-2a cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy SMARCC1 Ready-To-Use IHC Kit. SMARCC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86015-1-RR targets SMARCC1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SMARCC1 fusion protein Ag12109 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
别名 BAF155, BRG1-associated factor 155, CRACC1, Rsc8, SRG3
计算分子量 1105 aa, 123 kDa
观测分子量155-160 kDa
GenBank蛋白编号BC050564
基因名称 SMARCC1
Gene ID (NCBI) 6599
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92922
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMARCC1, also named as SWI/SNF complex 155 kDa subunit or BAF155, is a 1105 amino acid protein, which belongs to the SMARCC family. SMARCC1 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. SMARCC1 is Involved in transcriptional activation and repression of select genes by chromatin remodeling. SMARCC1 may stimulate the ATPase activity of the catalytic subunit of the complex. SMARCC1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calculated molecular weight of TCEA1 is 123 kDa, but modified SMARCC1 is about 155-160 kDa. (PMID: 24025402)

实验方案

Product Specific Protocols
WB protocol for SMARCC1 antibody 86015-1-RRDownload protocol
IF protocol for SMARCC1 antibody 86015-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols