• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 86015-1-RR (same clone as 86015-1-PBS)

    WB analysis using 86015-1-RR (same clone as 86015-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86015-1-RR (SMARCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86015-1-PBS in a different storage buffer formulation.

  • Sample test of MP02222-1

    Sample test of MP02222-1

    The mean SMARCC1 concentration was determined to be 130.2 ng/mL in Jurkat cell extract based on a 2.2 mg/mL extract load.

  • IF Staining of Jurkat using 86015-1-RR (same clone as 86015-1-PBS)

    IF Staining of Jurkat using 86015-1-RR (same clone as 86015-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SMARCC1 antibody (86015-1-RR, Clone: 250455A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86015-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02222-1

    Cytometric bead array standard curve of MP02222-1

    Cytometric bead array standard curve of MP02222-1, SMARCC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86015-1-PBS. Detection antibody: 86015-2-PBS. Standard: Ag12109. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 86015-1-RR

    Affinity and Kinetic Characterization of 86015-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86015-1-RR against Human SMARCC1 were performed. The affinity constant is 0.393 nM.

SMARCC1 Recombinant antibody, PBS Only (Capture)

SMARCC1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 86015-1-PBS

产品说明书

CloneNo. 250455A1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

别名

BAF155, BRG1-associated factor 155, CRACC1, Rsc8, SRG3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy SMARCC1 Ready-To-Use IHC Kit. SMARCC1 primary antibody included.

产品信息

86015-1-PBS targets SMARCC1 as part of a matched antibody pair:

MP02222-1: 86015-1-PBS capture and 86015-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 SMARCC1 fusion protein Ag12109 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
别名 BAF155, BRG1-associated factor 155, CRACC1, Rsc8, SRG3
计算分子量 1105 aa, 123 kDa
观测分子量155-160 kDa
GenBank蛋白编号BC050564
基因名称 SMARCC1
Gene ID (NCBI) 6599
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92922
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMARCC1, also named as SWI/SNF complex 155 kDa subunit or BAF155, is a 1105 amino acid protein, which belongs to the SMARCC family. SMARCC1 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. SMARCC1 is Involved in transcriptional activation and repression of select genes by chromatin remodeling. SMARCC1 may stimulate the ATPase activity of the catalytic subunit of the complex. SMARCC1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calculated molecular weight of TCEA1 is 123 kDa, but modified SMARCC1 is about 155-160 kDa. (PMID: 24025402)