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验证数据展示

  • IF Staining of HeLa using CL488-85018-4

    IF Staining of HeLa using CL488-85018-4

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SMARCA2-Specific antibody (CL488-85018-4, Clone: 242511E1 ) at dilution of 1:200, CL594-phalloidin (red).

CoraLite® Plus 488-conjugated SMARCA2-Specific Recombinant monoclonal antibody

SMARCA2-Specific Uni-rAb® Recombinant Antibody for IF/ICC
Cat No. CL488-85018-4

产品说明书

CloneNo. 242511E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

SMARCA2, ATP-dependent helicase SMARCA2, BAF190, BAF190B, BRG1-associated factor 190B

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SMARCA2 Ready-To-Use IHC Kit. SMARCA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 85018-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-85018-4 targets SMARCA2-Specific in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25157

Product name: Recombinant human SMARCA2-Specific protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-272 aa of NM_003070

Sequence: MSTPTDPGAMPHPGPSPGPGPSPGPILGPSPGPGPSPGSVHSMMGPSPGPPSVSHPMPTMGSTDFPQEGMHQMHKPIDGIHDKGIVEDIHCGSMKGTGMRPPHPGMGPPQSPMDQHSQGYMSPHPSPLGAPEHVSSPMSGGGPTPPQMPPSQPGALIPGDPQAMSQPNRGPSPFSPVQLHQLRAQILAYKMLARGQPLPETLQLAVQGKRTLPGLQQQQQQQQQQQQQQQQQQQQQQQPQQQPPQPQTQQQQQPALVNYNRPSGPGPELSGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
别名 SMARCA2, ATP-dependent helicase SMARCA2, BAF190, BAF190B, BRG1-associated factor 190B
计算分子量 181 kDa
观测分子量210 kDa
GenBank蛋白编号NM_003070
基因名称 SMARCA2
Gene ID (NCBI) 6595
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP51531
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SMARCA2-Specific antibody CL488-85018-4Download protocol
Standard Protocols
Click here to view our Standard Protocols
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