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验证数据展示

  • WB analysis using 85018-4-RR (same clone as 85018-4-PBS)

    WB analysis using 85018-4-RR (same clone as 85018-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85018-4-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 85018-4-RR (same clone as 85018-4-PBS)

    IHC staining of human placenta using 85018-4-RR (same clone as 85018-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85018-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMARCA2 antibody included
  • IHC staining of mouse brain using 85018-4-RR (same clone as 85018-4-PBS)

    IHC staining of mouse brain using 85018-4-RR (same clone as 85018-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85018-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMARCA2 antibody included
  • IHC staining of mouse brain using 85018-4-RR (same clone as 85018-4-PBS)

    IHC staining of mouse brain using 85018-4-RR (same clone as 85018-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85018-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMARCA2 antibody included
  • IF Staining of HeLa using 85018-4-RR (same clone as 85018-4-PBS)

    IF Staining of HeLa using 85018-4-RR (same clone as 85018-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMARCA2-Specific antibody (85018-4-RR, Clone: 242511E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85018-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85018-4-RR

    Affinity and Kinetic Characterization of 85018-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85018-4-RR against Human SMARCA2-Specific were performed. The affinity constant is 3.86 nM.

SMARCA2-Specific Recombinant monoclonal antibody, PBS Only

SMARCA2-Specific Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85018-4-PBS

产品说明书

CloneNo. 242511E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

SMARCA2, 242511E1, ATP-dependent helicase SMARCA2, BAF190, BAF190B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SMARCA2 Ready-To-Use IHC Kit. SMARCA2 primary antibody included.

产品信息

85018-4-PBS targets SMARCA2-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25157

Product name: Recombinant human SMARCA2-Specific protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-272 aa of NM_003070

Sequence: MSTPTDPGAMPHPGPSPGPGPSPGPILGPSPGPGPSPGSVHSMMGPSPGPPSVSHPMPTMGSTDFPQEGMHQMHKPIDGIHDKGIVEDIHCGSMKGTGMRPPHPGMGPPQSPMDQHSQGYMSPHPSPLGAPEHVSSPMSGGGPTPPQMPPSQPGALIPGDPQAMSQPNRGPSPFSPVQLHQLRAQILAYKMLARGQPLPETLQLAVQGKRTLPGLQQQQQQQQQQQQQQQQQQQQQQQPQQQPPQPQTQQQQQPALVNYNRPSGPGPELSGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
别名 SMARCA2, 242511E1, ATP-dependent helicase SMARCA2, BAF190, BAF190B
计算分子量 181 kDa
观测分子量210 kDa
GenBank蛋白编号NM_003070
基因名称 SMARCA2
Gene ID (NCBI) 6595
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP51531
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

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